Clarity OTX Pro Lysis Loading Buffer is a ready-to-use lysis and loading reagent formulated to prepare biological samples for Clarity OTX Pro solid-phase extraction. It lyses cells, denatures proteins, inhibits nucleases and proteases, and conditions samples for reliable SPE extraction and LC/MS analysis of therapeutic oligonucleotides and their metabolites.
- Provides cell lysis, liposome disruption, and protein denaturing.
- Inhibits nucleases and removes protease activity in biological fluids.
- Formulated for extraction of therapeutic oligonucleotides and metabolites from plasma, serum, urine, sputum, and tissue.
- Ready-to-use, 100 mL volume, pack size: each.
- Recommended sample ratios: at least 1:1 buffer to biological fluids; approximately 3-5x buffer to tissue; target pH ~5.5 after mixing.
- Contains Triton X-100 and guanidine hydrochloride; handle according to safety data sheet.